Hydrothermally green synthesized ginger derived carbon nanodots showing antioxidant, catalytic reducing and anticancer properties*AbstractCarbogenic carbon nanodots containing Curcuminoids and 6-gingerol layers with bulk of resonating non bonded electrons were synthesized using simple and green hydrothermal method from natural herb Ginger. As synthesized C nanodots were characterized using UV-Vis spectroscopy, IR, DLS, and TEM analysis. The antioxidant, catalytic reducing and anticancer properties of C dots were studied using ex vivo KMnO4 reduction assay, catalytic 4-nitrophenol reduction test, and in vitro MTT assay on MCF-7 cell line respectively. These carbogenic carbon nanoparticles shown quantum particle size of 4 nm. The green synthesized C dots shown excellent in vitro biological anti oxidant and anticancer properties along with reducing nature. This study exhibited the novelty of these green synthesized bioactive carbon nanodots for tagging and coating of bioactive materials for drug vectorization, biodetection, biocompatible cell targeting and biological applications.Keywords: Ginger Carbon Dots, Antioxidant, Reducing, Anticancer, MCF-7 cell line.1. IntroductionCarbon nanodots (CNDs), as a new member of carbon nanomaterial family, have aroused great interest because of their outstanding water solubility, high sensitivity and selectivity to target analytes, low toxicity, favorable biocompatibility, and excellent photo stability1-3. Lots of methods for the production of CNDs have been reported such as hydrothermal and solvothermal technology and needs simple equipments. Due to their excellent fluorescence, CNDs have made impressive strides in sensitivity and selectivity to a diverse array of salt ions, organic/biological molecules and target gases4-8. The development of CDs as nano probes is still in its infancy, but continued progress may lead to their integration into environmental and biological applications. CNDs mainly have two major categories as carbogenic and graphitic carbon nanodots. These carbon nanomaterials can be derived from natural resources, biomolecules as carbohydrates, proteins using hydrothermal, solvothermal and microwave synthetic methods. As synthesized polymeric layer structured CNDs contain N, S, O hetero atoms with Carbon as main elemental composition with SP2 hybridization and along with conjugation and plenty of mobile electrons. Carbogenic carbon quantum dots or CNDs can be derived from natural herbs and wastes and contain mainly SP2 hybridization and conjugation of Carbon atoms and or with S, N, O atoms. CNDs are conjugated systems which have sp2 and sp3 hybridized carbons atoms with plenty of oxygen containing groups. CNDs obtained by the hydrothermal treatment reaction contains ionization, condensation, polymerization, and carbonization by bottom-up method. Such carbon dots may contain plenty of mobile electrons in polymeric layers limiting in size of 2 to 8 nm. and can show excellent non blinking photoluminescence and UV-VIS absorption of radiations9-17. So these CNDs can be used for coating biomaterials, nano probes, nano vectors for bio applications18-22. Ginger is one of the most widely used herb condiments in the world and is used as a traditional medicinal herb in eastern countries like India, China, due to its antioxidative, anti-inflammatory, and anti-carcinogenic properties. Curcumin, a hydrophobic polyphenol [(1E,6E)-1,7-bis(4-hydroxy-3-methoxyphenyl) hepta-1,6-dienne-3,5-dione], is a yellow ingredient of ginger, which exhibits many biological activities such as antibacterial, anticancer, and hepatoprotective activities. Curcumin can inhibit the growth of the human cancer cells and change the cell-surface morphology, and trigger pro-apoptotic factor (e.g., mitochondrial damage and caspase activation) to promote cell apoptosis, with low toxicity to other cells. In addition to curcumin, 6-gingerol (a natural analog of curcumin; [5-hydroxy-1-(40-hydroxy-30-methoxyphenyl)3-decanone]) is another abundant constituent of ginger, which exhibits antimetastatic and anti-invasive pharmacological activities on cancer cells. Such active ingredients of ginger can be accommodated in quantum dot polymeric level by carbonization of ginger to CNDs for use of biological activities and applications. Here in this paper we have synthesized carbogenic carbon nanodots by use of natural herb ginger. After physicochemical characterization of these CNDs, their reducing nature, catalytic activities, biocompatibility, anti oxidant nature and anticancer potential have been checked by various biological screening tests.2. Materials and Methods2.1. MaterialsAll the chemicals used for synthesis of CNDs and their biological screening such as NaOH, KMnO4, 2,4-DNP, NaBH4, 4-Nitrophenol, Vitamin-C, 5-Fluorouracil, MTT reagent were of A. R. grade from S. D. fine chem. and Merck ltd. Cell culture medium DMEM, 10% fetal bovine serum, Human breast cancer cell line (MCF-7) were procured from NCCS center, Pune, India. The double distilled water from Millipore system was used throughout the synthesis and testing.2.2. Methods2.2.1. Hydrothermal synthesis of Carbogenic CNDs from gingerThe carbogenic carbon nanoparticles (CNDs) were synthesized using hydrothermal green method with some modification from natural herb ginger. In brief fresh tenders of rhizomes of ginger were purchased from local market and washed with boiled water. Then the surface cover of tenders peeled and cut into small pieces. These pieces were crushed by mortar and pestle and aqueous extract was taken in appropriate volume(25 ml.) in a beaker. 0.01 M NaOH (10 Ml.) and 15 ml. double distilled water was added in to beaker and basic extract was carbonized at 200oC for 3 Hours. over hot plate under air atmosphere. As formed carbon residue was diluted with double distilled water to 100 ml. and filtered through filter paper no.1. then finally the filtrate was dialyzed through dialysis membrane with porosity 2 nm. for 8 hrs. with stirring. The transparent brown liquid containing carbon nano dots stored in refrigerator for further use.2.2.2. Structural and morphological characterization of CNDsThe structure, hybridization, morphology, particle size and types of atoms of CNDs were confirmed on the basis of physicochemical characterization on the basis of UV-VIS and IR spectral analysis, TEM, DLS analysis. Systronic double beam spectrometer was used for UV-VIS spectral analysis of CNDs with solution conc. of 10 µg./ml. in water prepared after drying CNDs suspension at 100oC with water as blank. TEM image and DLS scattering for particle size of CNDs determined with original CNDs aqueous suspension. IR spectra of CNDs determined using KBr pallet method on Perkin Elmer series spectrometer.2.2.3. Reducing catalytic activity of CNDsThe reducing nature of CNDs along with catalytic activity was determined by reduction of 4-nitrophenol to 4-aminophenol in presence of CNDs with sodium borohydride (NaBH4). The role of CNDs on reduction rate was studied with UV-VIS spectrophotometry. The time required for reduction in presence of CNDs studied by wavelength scan spectra of 4-nitrophenol reduction to 4-aminophenol. Briefly, 2 ml. of 4-nitrophenol(0.01M) and 1 ml. NaBH4 (0.01M) with 1ml. water taken in cuvette and 1 ml. of 10 µg./ml. of CNDs added to this mixture. Suddenly UV-VIS spectra was recorded from 2 minutes after reaction up to 12 minutes. The online real time UV-VIS scan was performed until completion of reaction of 4-NP to 4-AP.2.2.4. Antioxidant property of CNDs by ex vivo KMnO4 assayThe antioxidant activity of CNDs were tested by ex vivo KMnO4 reduction assay with Vitamin-C as standard control antioxidant drug by UV-VIS spectrometer optometric absorbance measurement. Briefly 5 ml. 0.01M KMnO4 reacted with 5 ml. 1mg./ml. CNDs in a hard glass test tube sealed at open end with cotton and incubated in dark for 30 min. at 37oC in CO2 environment of incubator. The absorbance of bare potassium permanganate solution was determined before and after incubation with antioxidant material as OD1 and OD2 respectively. Same test was performed for CNDs and Std. Vitamin-C as control for various concentrations of 0.12, 0.25, 0.5 and 1 mg./ ml. finally the percent antioxidant activity of material determined by using ex vivo assay formula as,Percent antioxidant activity = OD1-OD2 / OD1 X 100 %2.2.5. Anticancer potential of CNDs by in vitro MTT assay on MCF-7Cancer cell cultures MCF-7 (human breast cancer) cell lines were purchased from NCCS, Pune, India. All cell lines were grown and maintained in suitable (DMEM -media and were grown and subcultured in medium supplemented with 10% fetal bovine serum,1% L-Glutamine.1% penicillin streptomycin antibiotic solution. All cells were trypsinated using trypsin-EDTA solution and seeded in 96- well plates.The newly synthesized CNDs were evaluated for their in vitro cytotoxic effects against MCF-7 (Breast cancer cell line), by the standard MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay using 5-FU (5-Fluorouracil) drug as a positive control in aqueous form.The MCF cell line was maintained in DMEM medium supplemented with 10 % fetal bovine serum. The cells were plated at a density of 1 × 105 cells per well in a 96-well plates, and cultured for 24 h at 37°C. The cells were subsequently exposed to 10 µM CNDs.The plates were incubated for 48 h, and cell proliferation was measured by adding 10µL of MTT (thiazolyl blue tetrazolium bromide) dye (5 mg ml-1 in phosphate-buffered saline) per well. The plates were incubated for a further 4 h at 37 °C in a humidified chamber containing 5% CO2. Formazan crystals formed due to reduction of dye by viable cells in each well were dissolved in 200 µl DMSO, and absorbance was read at 490 nm. The results were compared with the standard drug inhibitors 5 fluorouracil. (10µg/Ml.) Lastly percent cytotoxicity of CNDs was calculated by using following formula.Percent Cytotoxicity = Reading of control Reading of treated cells / Reading of control X 1003. Results and Discussion3.1. Morphological and structural characterization of carbon nanodots3.1.1. IR analysisThe IR spectra of CNDs shown peaks in both the regions of functional and fingerprint signals. The functional group signal region of spectra exhibited the presence of aromatic and conjugated OH groups, diketone, aromatic conjugated system, while fingerprint region of spectral signals shown presence of aromatic OCH3 aromatic-H, -CH2 stretch, and presence of sp2 hybridized carbon groups. The IR signal frequency at 3486 cm-1 is due to the aromatic OH groups. Peak at 2930 cm-1 show presence of conjugated diketonic group. Peak at 1644 cm-1 may be attributed to aromatic -OCH3. All other peaks of spectra in fingerprint area may be due to presence of conjugated CH groups, aromatic-H, -C=O etc. (Fig.1). hence all these evidences prove the presence of curcuminoids and 6-gingerol layer of CNDs. Hence these CNDs are carbogenic carbon nanoparticles containing sp2 hybridized conjugated carbon atoms containing plenty of Pi and non bonded electrons with chain sizes in nanometer range.3.1.2. UV-VIS absorption spectra of CNDsThe UV-VIS spectra of CNDs shows two absorption peaks at 210 nm. and 315 nm. and with long tailing in visible spectra. These observations clearly indicates the presence of Pi and non bonding electrons in carbogenic CNDs. The absorption peak at 210 nm. shows n to ?* transition and peak at 315 is due to ? to ?* transition and electron radiation relaxation (Fig. 2). Hence these spectral analysis indicates the presence of conjugated carbon system with n and ? electrons probably due to curcuminoids and 6-gingerol in CNDs with SP2 hybridized carbon in conjugation. So CNDs could contain aromatic conjugated natural carotenoid like diketonic molecular systems of these active ingredients of ginger.The DLS scattering spectra of CNDs reveal that, the average particle size of the carbon nanoparticles is 4 nm. which matched with the TEM image of CNDs and with size of these carbon quantum nanoparticles. The sizes of CNDs varies from 2 to 12 nm. (Fig. 3), but maximum CNDs shows size between 2 to 6 nm. hence these are quantum dot carbon nanoparticles with abundance of mobile electrons responsible for light scattering in DLS and electron scattering in TEM. The TEM image of CNDs proved that there is some aggregation showing amorphous nature and circular morphology of CNDs material.3.2.1. Reducing catalytic activity of CNDs by reduction of 4-NP to 4-APThe reducing and catalytic nature of CNDs tested by reduction of 4-nitrophenol(4-NP) to 4-aminophenol(4-AP) in presence of NaBH4 by absorbance measurement with time lag of reaction. The initial absorption spectra of 4-NP and peak at 330 nm. vanished after reduction by CNDs in presence of NaBH4 as hydrogen source and CNDs as catalyst. After 12 min. 4-NP is totally converted to 4-AP showing new absorption spectra with peak at 460 nm. the catalytic reduction cycle of reaction completes after 12 min. UV-VIS real time online reaction wavelength scan performed from 2 min. to 12 min. until completion of reaction in cuvette. The formation of 4-AP take place with shifting and dampening of peak of 4-NP and formation of new peak of 4-AP in UV-VIS wavelength scan spectra (Fig.4).Very good antioxidant activity is shown by CNDs derived from natural herb ginger compared with Vitamin-C by ex- vivo KMnO4 assay. The antioxidant activity determined for 0.12, 0.25, 0.5 and 1 mg./ml. concentrations of CNDs and Vit.-C as standard control is represented in Fig.5. The CNDs shows higher antioxidant activity than Vit.-C which increases with increase in concentration of drug. The color of KMnO4 fade after treatment of material and incubation in biological environment conditions, which elaborates the reducing as well as antioxidant nature of control Vit.-C and material CNDs. EC50 minimal inhibition concentration value or half reducing antioxidant activity of Vit.-C and CNDs determined by triplicate absorbance measurement are 0.62 and 0.48 mg./ml. respectively. So CNDs are better option for antioxidant material than Vit.-C for bio applications with minimum concentration for high antioxidant activity.3.2.3. Anticancer potential of CNDs by in vitro MTT assay on MCF-7 cell lineMTT assay performed on MCF-7 human breast cancer cell line for anticancer potential of CNDs with 5-Fluorouracil as control drug shows moderate to good activity against these cells. As CNDs are reducing, antioxidant and contain free mobile electrons they can inhibit growth of MCF-7 by generation of ROS (Reactive Oxygen Species) at acidic pH inside cells. The cell viability of MCF-7 decreased by CNDs up to 64 % at concentration of 5 µg./ml. in sterile phosphate buffer saline with pH=7.4 and up to 78 % at 10 µg./ml. compared with 5-FU to 15 % at 5 µg./ml. and 19 % at 10 µg./ml. respectively. So the EC50 = 5 µg,/ml. shown by CNDs prove that a good candidate drug for anticancer application on selected cell lines without toxicity on normal cells.4. ConclusionThe green, hydrothermal and cost effective synthesis of carbon nano dots from natural herb ginger has been reported in this paper. The synthesized CNDs had shown presence of layers of curcuminoids and 6-gingerol as drug ingredients from ginger with conjugation and plenty of mobile electrons. These CNDs had explored reducing catalytic, antioxidant, anticancer activities. Hence these carbogenic CNDs with 4 nm. mean quantum size exhibited important biological activities. So these water soluble CNDs derived from natural herb and in basic medium by carbonization process can be used as tagging and coating material on bioactive nanomaterials for cell vectorization or probing and for biocatalytic, antioxidant and anticancer applications. Overall the CNDs derived from natural herb ginger exhibit characteristics of:better reducing, catalytic, antioxidant and anticancer activitiesgood water and phosphate buffer solubility hence these are bioavailable drug candidategood stability and low toxicity on normal cells and moderate cytotoxicity on cancer cellsquantum dot size and presence of curcuminoids and or 6-gingerol natural multiactive drugsSo the CNDs can be better options for toxic quantum dot materials as CdS, and CdSe in biological applications.Get Help With Your EssayIf you need assistance with writing your essay, our professional essay writing service is here to help!Find out more
Hydrothermally Green Synthesized Ginger Properties
Mar 18, 2020 | Sciences
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- Charles R Drew University of Medicine and Science
- charles sturt university
- Chaudhary Charan Singh University, Humanities
- CHEM101
- CHEM111
- CHEM1411, Science
- CHEM202, Science
- CHEM210, Science
- CHEM410
- Chemistry
- Chemistry – Chemical Engineering
- Chemistry – Organic chemistry
- Chemistry – Pharmacology
- Chemistry – Physical chemistry
- ChemistryChemistry
- Childcare
- CHMY373, SCIENCE
- Choose three problematic issues that are currently facing older people living in the community?
- CINE286U
- CIS210
- cis273
- CIS359
- CIS510, Other
- CIS524
- CIVL6603, Science
- CJ430, SCIENCE
- CJA444
- CJUS300, Other
- Classics
- CMIT495
- CMSC140
- Colorado Christian University
- COLORADO STATE UNIVERSITY GLOBAL, SCIENCE
- COLORADO STATE UNIVERSITY, SCIENCE
- Colorado State UniversityGlobal
- Colorado Technical University
- COLORADO TECHNICAL UNIVERSITY, OTHER
- Colorado Technical University, Programming
- Columbia Southern University
- COLUMBIA SOUTHERN UNIVERSITY, OTHER
- Columbia Southern University, Science
- COLUMBIA SOUTHERN UNIVERSITY, WRITING
- Commerce
- Communication
- Communications
- COMMUNITY-BASED CORRECTIONS
- COMP1007
- Computer Science
- Computer Science – Java
- Computer Science- Python
- Computer ScienceComputer Science
- Construction
- correctness
- Cosc1437
- counseling chemical dependency adolescents
- Criminology
- CRJ305
- cross-site scripting
- CS101
- CSIT 100, PROGRAMMING
- CSPM326
- CST 610, PROGRAMMING
- Cultural Studies
- culturally appropriate intervention to address childhood obesity in a low-income African American community.
- CUR535
- CUYAMACA COLLEGE, HUMANITIES
- CUYMACA COLLAGE, HUMANITIES
- Data Analysis
- DAVIDSON COLLEGE, OTHER
- defensive programming allows for more efficient processes while also protecting systems from attack.
- DES201
- Describe the difference between glycogenesis and glycogenin ? Explain in 10 to 12 lines.
- Design
- Digital Marketing Plan for Nissan Motor Co. The plan will identify the current marketing opportunity and/or problem(s) and propose digital marketing solutions. Please use header in the attachment."
- Discuss one way in which the Soviet Union fulfilled communist thought, and another way in which it did not with reference to O'Neil's Chapter 9.
- Discuss the pros and cons of free-market based economies and how they impact the modern, globalized economy? What comes to your mind when you hear the term "globalization?"
- Dissertation
- DMM612, Science
- DMM649, SCIENCE
- Draft and essay of 1,000 words minimum, stating the Most Important and Relevant aspects to be considered when carrying on INTERNATIONAL NEGOTIATIONS or MULTI-CULTURAL NEGOTIATIONS.
- Drama
- each time a user extracts the ZIP file
- Earth Science – Geography
- Earth Science – GeographyEarth Science – Geography
- Earth Science – Geology
- EAS1601
- ECD 541, HUMANITIES
- ECE 452
- Ecommerce
- ECON335
- Economics
- ECPI University
- EDUC696
- Education
- EducationEducation
- EEL3472C
- EEL3705
- EET110
- EFFAT UNIVERSITY JEDDAH, HUMANITIES
- elasticity
- ELI2055A
- EMDG 230, SCIENCE
- Emglish
- Emory University
- Employment
- EN106
- EN106, HUMANITIES
- EN109
- EN206, HUMANITIES
- ENC1102, Writing
- eng 100
- ENG100
- ENG101
- ENG101, Humanities
- ENG102
- ENG102, Humanities
- eng106
- ENG1102, WRITING
- ENG124
- ENG124, Humanities
- ENG124, Writing
- ENG1340, HUMANITIES
- ENG200, Humanities
- ENG207
- eng2206
- ENG2211
- ENG305
- ENG812
- Engineering
- Engineering – Chemical Engineering
- Engineering – Civil Engineering
- Engineering – Civil EngineeringEngineering – Civil Engineering
- Engineering – Electrical Engineering
- Engineering – Electrical EngineeringEngineering – Electrical Engineering
- Engineering – Electronic Engineering
- Engineering – Mechanical Engineering
- Engineering – Mechanical EngineeringEngineering – Mechanical Engineering
- Engineering – Telecommunications Engineering
- EngineeringEngineering
- ENGL 120
- ENGL 124, OTHER
- ENGL 124, WRITING
- ENGL 2030, HUMANITIES
- ENGL1102
- ENGL120, HUMANITIES
- ENGL120SP2019, WRITING
- ENGL126
- ENGL1302
- ENGL130E, HUMANITIES
- ENGL147N, HUMANITIES
- ENGL2, Humanities
- English
- English – Article writing
- English – Article writingEnglish – Article writing
- English Language
- English Literature
- EnglishEnglish
- ENGR350
- ENST202CORE274
- ensuring you allocate dedicated time for coursework
- Environment
- Environmental Science
- Environmental Sciences
- Environmental Studies
- especially for a small company
- Essay Writing
- etc¦). Please note at least five organizational activities and be specific when responding.
- ETH321
- ETHC445N
- Ethnic Studies 101
- European Studies
- EXNS6223
- Family
- Fashion
- February 20). What is defensive programming? EasyTechJunkie. Retrieved December 30
- film industry
- FILM INDUSTRY, HUMANITIES
- Film Studies
- FIN 500
- FIN330, MATHEMATICS
- FIN370
- Final Essay
- Find the uniform most powerful level of alpha test and determine sample size with the central limit theorem
- Florida International University
- Florida National University
- Florida State College at Jacksonville
- FoothillDe Anza Community College District
- Foreign Languages
- Foreign Languages – Spanish
- formulations
- from https://www.pcmag.com/news/fat32-vs-ntfs-choose-your-own-format
- G124/enc1101
- Gallaudet University
- General Studies
- General_Business
- GEO1206
- GEOG100, Science
- Geography
- GEOL3200, HUMANITIES
- Geometry
- George Mason University
- GERM1027
- GERO 101, SCIENCE
- GERON101
- GLG101, Science
- GO16
- Government
- GovernmentGovernment
- GOVT2305
- GOVT2305, Humanities
- GOVT2306
- Grand Canyon University, Science
- Grand Canyon University, Writing
- Grantham University
- GRANTHAM UNIVERSITY, PROGRAMMING
- GRANTHAM UNIVERSITY, WRITING
- GROSSMONT COLLEGE, HUMANITIES
- Grossmont-Cuyamaca Community College District
- GROSSMONT-CUYAMACA COMMUNITY COLLEGE DISTRICT, HUMANITIES
- GU299, WRITING
- Hawaii Pacific University
- HC310
- HCA415
- HCA521
- HCM550, SCIENCE
- Hcs370
- HCS446
- he focused on aspects of the U.S. that combined democratic and increasingly capitalist characteristics. THINK ABOUT the points De Tocqueville made.
- Health & Medical
- Healthcare
- HIM 2588, MATHEMATICS
- HIM 500, SCIENCE
- HIM301
- HIS 108
- HIS101
- HIS105
- HIS200
- HIST104A, Humanities
- HIST111
- HIST1301, HUMANITIES
- HIST1302
- HIST1320
- HIST1700
- HIST2620
- HIST350, Humanities
- HIST405N, HUMANITIES
- HIST459, Humanities
- History
- History – American history
- History – American historyHistory – American history
- History – Ancient history
- History – Ancient historyHistory – Ancient history
- History – World history
- History – World historyHistory – World history
- HISTORY4250, Humanities
- HistoryHistory
- HLSS508, OTHER
- HMP403
- Hospitality
- HOST1066, WRITING
- Housing
- How do the changes in ship technology effect port operations? Discuss at least 3 factors contributing to port operations and development. Address cargo and passenger liners.250 words
- How have Mary Calderone, SIECUS and other sex educators changed how sex education is perceived? (100 words minimum)
- HOWARD UNIVERSITY, SCIENCE
- HR Management
- HRM300
- HRT6050, Writing
- HSA305
- HSA535
- HSC3201
- HSN476
- HUM1002
- HUM115
- HUM115, Writing
- Human Resource
- Human Resource Management
- Human Resource ManagementHuman Resource Management
- Human Resources
- HUMAN RESOURCES DEVELOPMENT AND MANAGEMENT, SCIENCE
- Human Rights
- HUMANITIES
- Humanities, Alcorn State University
- HUMANITIES, HY 1110
- Humanities, LMC3225D
- HUMANITIES, LONG BEACH CITY COLLEGE
- HUMANITIES, MUSIC1306
- HUMANITIES, OAKLAND COMMUNITY COLLEGE
- HUMANITIES, PH 100
- HUMANITIES, POINT LOMA NAZARENE UNIVERSITY
- HUMANITIES, PRINCE GEORGE'S COMMUNITY COLLEGE
- Humanities, PSY105
- HUMANITIES, PSY330 THEORIES OF PERSONALITY
- Humanities, PSYC 1101
- HUMANITIES, PSYCH305
- HUMANITIES, PSYCH635 PSYCHOLOGY OF LEARNING
- HUMANITIES, RSCH8110
- HUMANITIES, SAN DIEGO STATE UNIVERSITY
- HUMANITIES, SAN JACINTO COLLEGE
- Humanities, SOC1010
- HUMANITIES, SOC401
- HUMANITIES, SOCIOLOGY OF RELIGION
- HUMANITIES, SOUTHERN NEW HAMPSHIRE UNIVERSITY
- HUMANITIES, STRAYER UNIVERSITY
- HUMANITIES, SWK110
- HUMANITIES, UNIVERSITY OF CALIFORNIA
- HUMANITIES, UNIVERSITY OF CALIFORNIA IRVINE
- HUMANITIES, UNIVERSITY OF HOUSTON-DOWNTOWN
- Humanities, University of Maryland University College
- i need the attached work to look like this. please redo and make it look like this.
- I need these questions answered fully. I have the assignment and the notes attached for it. Do not use chegg or course hero. This is due Wednesday 4/14 at 10:00 pm which is almost 4 full days. Thanks!
- I need to re organize a research paper I attached all my information and I attached you an example how is going to be. Please follow the instruction and the references has to be APA 7edition
- Identify a cardiac or respiratory issue and outline the key steps necessary to include for prevention and health promotion
- identify the leadership theory that best aligns with your personal leadership style
- if you suggest trying to do this
- IGLOBAL UNIVERSITY
- IHS2215
- Iii Mlch
- III nuistery of such superficialities was meant to
- Implement classifiers KMeans, Random Forest and Decision Tree, SVM,XGBoost and Naive Bayes for the given dataset of audio samples to findout top genre for an audio sample(which one fits best)
- In a cardiac issue what are the key steps necessary to include for prevention and health promotion.
- in any other way
- include a paragraph about which side of the case a forensic psychologists might support and why.
- indeed
- India
- INDIANA UNIVERSITY BLOOMINGTON, SCIENCE
- INF690
- INF690, Other
- Information Systems
- Information SystemsInformation Systems
- Information Technology
- INSTITUTE OF PUBLIC ADMINISTRATION SAUDI ARABIA, PROGRAMMING
- INT700, OTHER
- International Business
- International Relations
- International Studies
- Internet
- Introduction to Biology
- Is jury nullification sometimes justifiable? When?
- ISSC351
- It Research
- IT380
- IT550, Business & Finance , Southern New Hampshire University
- ITC3001
- ITP120
- ITS 631, PROGRAMMING
- ITS835, Other
- JEDDAH COLLEGE OF ADVERTISING, WRITING
- Journalism
- KNOWLEDGE IS POWER, OTHER
- Languages
- Law
- Law – Civil
- Law – CivilLaw – Civil
- Law – Criminal
- Law – CriminalLaw – Criminal
- LawLaw
- Leadership
- lecture slides
- Leisure Management
- Liberty University
- LIBERTY UNIVERSITY, WRITING
- lIlgll.Igt· (1II1In·d
- Linguistics
- literacy is always plural: literacies (there are many of them
- Literature
- Literature Review
- Literature review funnel on "cyber security"
- LiteratureLiterature
- MA105
- MAJAN COLLEGE, WRITING
- Management
- Manpower
- Marketing
- Math
- MATH 1030
- MATH144, MATHEMATICS
- Mathematics
- Mathematics – Algebra
- Mathematics – Calculus
- Mathematics – Geometry
- Mathematics – Numerical analysis
- Mathematics – Precalculus
- Mathematics – Probability
- Mathematics – Statistics
- Mathematics – StatisticsMathematics – Statistics
- Mathematics – Trigonometry
- MATHEMATICS, MGT3332
- Mathematics, National American University
- Mathematics, PSY325
- MATHEMATICS, PUBH8545
- Mathematics, QNT275
- MATHEMATICS, STAT 201
- MBA503
- McMaster University
- ME350B, SCIENCE
- MECH4430, SCIENCE
- Mechanics
- Media
- Medical
- Medical Essays
- MGMT2702
- MGMT410
- MGT173, SCIENCE
- MHR6451
- MIAMI UNIVERSITY, WRITING
- Military
- Military Science
- MKT501
- MKT690, OTHER
- MN576
- MN581
- MN610, SCIENCE
- MNGT3711
- Music
- MVC109
- N4685
- NATIONAL INSTITUTES OF HEALTH, SCIENCE
- NATIONAL UNIVERSITY, SCIENCE
- Needs to be at least 300 wordswithin the past five years.No plagiarism! What key aspects do you believe should guide ethical behavior related to health information, technology, and social media?
- no workable ""affirmative action"" for Discourses: you can't 19 Ill' let into the game after missing the apprenticeship and be expected to have a fnir shot at playing it. Social groups will not
- Northcentral University
- not writing)
- nothing can stand in her way once she has her mind set. I will say that she can sometimes be hard headed
- Nova Southeastern University
- NR447, SCIENCE
- NRS429VN
- NRS44V, OTHER
- NRS451VN
- NRSE4540
- NSG426
- NSG486
- NSG6102
- NSG6102, SCIENCE
- Numerical Analysis
- NUR231NUR2349, SCIENCE
- NUR647E
- NURS350
- NURS508
- NURS6640
- Nursing
- NURSING LEADERSHIP AND MANAGEMENT, SCIENCE
- NursingNursing
- Nutrition
- offering learners the flexibility to acquire new skills and knowledge from the comfort of their homes. However
- OHIO UNIVERSITY, SCIENCE
- Online Discussion Forums Grade and Reflection Assignment : Current Topic Artificial Intelligence HR Planning Career and Management Development Labour RelationsForum
- operation security
- Operations Management
- or do those companies have an ethical obligation to protect people? In this assignment
- ORG5800, OTHER
- Organisations
- OTHER
- Other, PAD631
- OTHER, PARK UNIVERSITY
- OTHER, PLA1223
- Other, POLI330N
- OTHER, PROFESSIONAL NURSING NU231 NUR2349
- Other, RTM404
- OTHER, SAINT LEO UNIVERSITY
- OTHER, SOC3210C1
- Other, SOCW6333
- OTHER, SOUTHERN NEW HAMPSHIRE UNIVERSITY
- Other, The University Of Southern Mississippi
- OTHER, TRIDENT UNIVERSITY INTERNATIONAL
- Other, UC
- OTHER, UNIVERSITY OF MARYLAND UNIVERSITY COLLEGE
- OTHER, UNIVERSITY OF SOUTH FLORIDA
- Other, Walden University
- paying attention to grammar and spelling. Actively participate in discussions
- Personal Development
- PhD Dissertation Research
- PHI 413V, SCIENCE
- Philosophy
- Photography
- PHY290
- PHYS204L206
- Physics
- Physics – Astronomy
- Physics – Electromagnetism
- Physics – Geophysics
- Physics – Mechanics
- Physics – Optics
- PhysicsPhysics
- Physiology
- PNGE332, SCIENCE
- Political Science
- Political SciencePolitical Science
- Politics
- PowerPoint slides
- privacy
- PROFESSIONAL NURSING NU231 NUR2349, SCIENCE
- PROFESSIONAL NURSING NU231NUR2349, SCIENCE
- Programming
- Programming , College of Applied Sciences
- PROGRAMMING, STRAYER UNIVERSITY
- PROGRAMMING, WILMINGTON UNIVERSITY
- Project Management
- proper grammar
- Protein
- provide a discussion on what could have been done better to minimize the risk of failure. If you have not yet been involved with a business process redesign
- PSYC8754, WRITING
- Psychology
- PsychologyPsychology
- PUB373, SCIENCE
- Purdue University
- Rasmussen College
- Read a poam and write a paragraph to prove "The table turned".
- Reading
- ReadingReading
- readings
- Reference this
- REL1030
- Religion
- RES861, Science RES861
- Research Methodology
- Research methods
- Research Proposal
- Research questions
- Retail
- Rutgers university
- SAFE4150
- safety statutes
- Santa Clara University
- SCI 220, SCIENCE
- SCI115, SCIENCE
- Science
- Science, Strayer University
- SCIENCE, THOMAS JEFFERSON UNIVERSITY
- SCIENCE, WEST COAST UNIVERSITY
- SCIENCE, WEST TEXAS A & M UNIVERSITY
- Sciences
- SCM371, Writing
- Search in scholarly sources the similarities and difference between PhD and DNP. Post three similarities and three differences found on your research. Do not forget to include your reference.
- Security
- self-actualization
- several things can happen
- Should the government operate public transportation systems?250 words
- so that it is not biased?
- so too
- SOC 450
- Social Policy
- Social Science
- Social Science – Philosophy
- Social Science – PhilosophySocial Science – Philosophy
- Social Science – Sociology
- Social Science – SociologySocial Science – Sociology
- Social Sciences
- Social ScienceSocial Science
- Social Work
- Society
- Sociology
- someone cannot engage in a Discourse in a less than fully fluent manner. You are either in it or you're not. Discourses are connected with displays of
- SP19, WRITING
- SPC2608
- SPD310
- Sports
- Statistics
- succeeding in online courses requires a different approach compared to traditional classroom settings. To help you make the most of your online learning experience
- such as notifications from social media or email. Organize your study materials and have a reliable internet connection to ensure seamless access to course materials.
- Technology
- that personal ethics and organizations ethics are two different and unrelated concepts. Others
- the attribute is useful
- The directions are attached. However you must read the PDF file first in order to answer the questions.
- the role of work and money
- Theatre
- then reply to a minimum of 2 of your classmates' original posts.
- Theology
- Threat of artificial intelligence 800 words.
- to be true of second language acquisition or socially situ ated cognition (Beebe
- to better promote the value and dignity of individuals or groups and to serve others in ways that promote human flourishing.
- to usc a Discourse. The most you can do is III It'! them practice being a linguist with you.
- total fat consumption
- Tourism
- Translation
- Transportation
- U110
- Uncategorized
- University of Central Missouri
- University of South Florida
- UNIVERSITY OF SOUTH FLORIDA, WRITING
- Video Games
- Watch this meditation https://www.youtube.com/watch?v=doQGx4hdF3M&feature=youtu.be and write a one page reflection
- WCWP10B
- we can always ask about how much ten- 12 """""" or conflict is present between any two of a person's Discourses (Rosaldo
- What approaches to the study of poverty does economic sociology offer? More specifically, what might sociologists studying poverty focus on besides poor households, neighborhoods, and individuals?
- What is the philosophical matrices for Behaviorism, Constructivism, and Reconstructivism
- What key aspects do you believe should guide ethical behavior related to health information, technology, and social media?
- what place did government have to improve markets? What does the concept of ""crowding out"" mean in all of this?
- What should be done to maintain optimum stock levels and why is it important to keep accurate and up-to-date records of stock in medical practice?
- whether good or bad. The intent of the short research projects is to dig a little deeper into some of the topics
- which triggers the vulnerability. As soon as the user downloads this shortcut file on Windows 10; windows explorer will
- Would somebody read these quotes and answer those three questions at the bottom for me?Disregard number two I will look for myself in the text.
- Write short essay based on evidence about the 2 cons of Sex Education 250-300 words 2 reference minimum no plagiarism
- WRITING
- writing assignment, you will analyze asymmetric and symmetric encryption. Evaluate the differences between the two of them and which one that you would determine is the most secure.
- Writing, Personal Code of Technology Ethics
- you believe you can provide the CIO with the information he needs.
- you will learn how to search for scholarly
- you will need to read the TCP standard. TCP was first defined in RFC 793. A link to this document is provided. https://tools.ietf.org/html/rfc793
- Young People
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